› 2j0y

X-ray diffraction
2.35Å resolution

L-ficolin complexed to b-1,3-D-glucan

Released:
DOI: 10.2210/pdb2j0y/pdb
PDB entry 2j0y coloured by chain, front view.
PDB entry 2j0y coloured by chain, side view.
PDB entry 2j0y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the innate immune recognition specificities of L- and H-ficolins.
Garlatti V, Belloy N, Martin L, Lacroix M, Matsushita M, Endo Y, Fujita T, Fontecilla-Camps JC, Arlaud GJ, Thielens NM, Gaboriaud C
EMBO J 26 623-33 (2007)
PMID: 17215869

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172309 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Ficolin-2 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 218 amino acids
Theoretical weight: 24.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q15485 (Residues: 96-313; Coverage: 76%)
Gene names: FCN2, FCNL
Sequence domains: Fibrinogen beta and gamma chains, C-terminal globular domain
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
4 bound ligands:
Ligand ACT 1 x ACT
Ligand CA 6 x CA
Ligand NAG 2 x NAG
Ligand BGC 1 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P32
Unit cell:
a: 96.08Å b: 96.08Å c: 140.98Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.196 0.242
Expression system: Spodoptera frugiperda

Quick links

Citations

35 review citations

An integrated view of humoral innate immunity: pentraxins as a paradigm.
Bottazzi et al. (2010)
34 more

6 mentions without citation

Structural insights into the innate immune recognition specificities of L- and H-ficolins.
Garlatti et al. (2007)
5 more