› 2jv1

Solution NMR

NMR structure of human insulin monomer in 35% CD3CN zinc free, 50 structures

Released:
DOI: 10.2210/pdb2jv1/pdb
PDB entry 2jv1 coloured by chain, ensemble of 50 models, front view.
PDB entry 2jv1 coloured by chain, ensemble of 50 models, side view.
PDB entry 2jv1 coloured by chain, ensemble of 50 models, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human insulin monomer in water/acetonitrile solution.
Bocian W, Sitkowski J, Bednarek E, Tarnowska A, Kawecki R, Kozerski L
J Biomol NMR 40 55-64 (2008)
PMID: 18040865

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134182 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing, molecular dynamics
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The critical role of mobile phase composition in size exclusion chromatography of protein pharmaceuticals.
Arakawa et al. (2010)
1 more

10 mentions without citation

Misfolded proteins in Alzheimer's disease and type II diabetes.
DeToma et al. (2012)
9 more