› 2k33

Solution NMR

Solution structure of an N-glycosylated protein using in vitro glycosylation

Released:
DOI: 10.2210/pdb2k33/pdb
PDB entry 2k33 coloured by chain, ensemble of 20 models, front view.
PDB entry 2k33 coloured by chain, ensemble of 20 models, side view.
PDB entry 2k33 coloured by chain, ensemble of 20 models, top view.
Source organism: Campylobacter jejuni
Primary publication:
NMR structure determination of a segmentally labeled glycoprotein using in vitro glycosylation.
Slynko V, Schubert M, Numao S, Kowarik M, Aebi M, Allain FH
J Am Chem Soc 131 1274-81 (2009)
PMID: 19154179

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186044 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
RND efflux pump membrane fusion protein barrel-sandwich domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 116 amino acids
Theoretical weight: 12.75 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RTE5 (Residues: 61-210; Coverage: 32%)
Gene names: E8P16_04140, E8P16_10560, GNO00_03940, QQI97_001038, cmeA
Sequence domains: Barrel-sandwich domain of CusB or HlyD membrane-fusion

Ligands and Environments

Carbohydrate polymer : NEW Components: B6D, A2G, BGC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Protein glycosylation in bacteria: sweeter than ever.
Nothaft et al. (2010)
13 more

2 mentions without citation

Architecture and roles of periplasmic adaptor proteins in tripartite efflux assemblies.
Symmons et al. (2015)
1 more