› 2kr4

Solution NMR

U-box domain of the E3 Ubiquitin Ligase E4B

Released:
DOI: 10.2210/pdb2kr4/pdb
PDB entry 2kr4 coloured by chain, ensemble of 20 models, front view.
PDB entry 2kr4 coloured by chain, ensemble of 20 models, side view.
PDB entry 2kr4 coloured by chain, ensemble of 20 models, top view.
Source organism: Mus musculus
Primary publication:
Structural and functional characterization of the monomeric U-box domain from E4B.
Nordquist KA, Dimitrova YN, Brzovic PS, Ridenour WB, Munro KA, Soss SE, Caprioli RM, Klevit RE, Chazin WJ
Biochemistry 49 347-55 (2010)
PMID: 20017557

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190182 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin conjugation factor E4 B Chain: A
Molecule details ›
Chain: A
Length: 85 amino acids
Theoretical weight: 9.78 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ES00 (Residues: 1092-1173; Coverage: 7%)
Gene names: Ube4b, Ufd2, Ufd2a
Sequence domains: U-box domain
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing, molecular dynamics
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The pathophysiology of defective proteostasis in the hypothalamus - from obesity to ageing.
Cavadas et al. (2016)
1 more

2 mentions without citation

Structure of an E3:E2~Ub complex reveals an allosteric mechanism shared among RING/U-box ligases.
Pruneda et al. (2012)
1 more