› 2kyi

Solution NMR

Solution NMR structure of Dsy0195(21-82) protein from Desulfitobacterium Hafniense. Northeast Structural Genomics Consortium Target DhR8C

Released:
DOI: 10.2210/pdb2kyi/pdb
PDB entry 2kyi coloured by chain, ensemble of 20 models, front view.
PDB entry 2kyi coloured by chain, ensemble of 20 models, side view.
PDB entry 2kyi coloured by chain, ensemble of 20 models, top view.
Source organism: Desulfitobacterium hafniense Y51
Primary publication:
Combining NMR and EPR methods for homodimer protein structure determination.
Yang Y, Ramelot TA, McCarrick RM, Ni S, Feldmann EA, Cort JR, Wang H, Ciccosanti C, Jiang M, Janjua H, Acton TB, Xiao R, Everett JK, Montelione GT, Kennedy MA
J Am Chem Soc 132 11910-3 (2010)
PMID: 20698532

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-557605 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sporulation protein YabP Chains: A, B
Molecule details ›
Chains: A, B
Length: 71 amino acids
Theoretical weight: 8 KDa
Source organism: Desulfitobacterium hafniense Y51
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q251Q8 (Residues: 20-82; Coverage: 65%)
Gene name: DSY0195
Sequence domains: YabP family
Structure domains: Immunoglobulin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 97%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

NMR approaches for structural analysis of multidomain proteins and complexes in solution.
Göbl et al. (2014)
4 more

5 mentions without citation

A community resource of experimental data for NMR / X-ray crystal structure pairs.
Everett et al. (2016)
4 more