› 2kym

Solution NMR

Solution structure of the Bem1p SH3-CI domain from L.elongisporus in complex with Ste20p peptide

Released:
DOI: 10.2210/pdb2kym/pdb
PDB entry 2kym coloured by chain, ensemble of 20 models, front view.
PDB entry 2kym coloured by chain, ensemble of 20 models, side view.
PDB entry 2kym coloured by chain, ensemble of 20 models, top view.
Source organisms:
Entry authors: Gorelik M, Muhandiram R, Davidson AR

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107556 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bud emergence protein 1 Chain: A
Molecule details ›
Chain: A
Length: 120 amino acids
Theoretical weight: 13.77 KDa
Source organism: Lodderomyces elongisporus NRRL YB-4239
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A5E3P5 (Residues: 2-117; Coverage: 26%)
Gene name: LELG_04233
Sequence domains: SH3 domain
Structure domains: SH3 Domains
Serine/threonine-protein kinase STE20 Chain: B
Molecule details ›
Chain: B
Length: 16 amino acids
Theoretical weight: 1.64 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: Q03497 (Residues: 468-483; Coverage: 2%)
Gene names: STE20, YHL007C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 88%
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links