› 2l5y

Solution NMR

NMR structure of calcium-loaded STIM2 EF-SAM.

Released:
DOI: 10.2210/pdb2l5y/pdb
PDB entry 2l5y coloured by chain, ensemble of 20 models, front view.
PDB entry 2l5y coloured by chain, ensemble of 20 models, side view.
PDB entry 2l5y coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Auto-inhibitory role of the EF-SAM domain of STIM proteins in store-operated calcium entry.
Zheng L, Stathopulos PB, Schindl R, Li GY, Romanin C, Ikura M
Proc Natl Acad Sci U S A 108 1337-42 (2011)
PMID: 21217057

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192825 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Stromal interaction molecule 2 Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 17.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9P246 (Residues: 62-205; Coverage: 20%)
Gene names: KIAA1482, STIM2
Sequence domains: SAM domain (Sterile alpha motif)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this NMR entry.
Chemical shift assignment: 72%
Refinement method: torsion angle dynamics, DGSA-distance geometry simulated annealing
Expression system: Escherichia coli

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Citations

47 review citations

Store-Operated Calcium Channels.
Prakriya et al. (2015)
46 more

5 mentions without citation

Structural aspects of calcium-release activated calcium channel function.
Stathopulos et al. (2013)
4 more