2l6u

Solution NMR

Solution NMR Structure of Med25(391-543) Comprising the Activator-Interacting Domain (ACID) of Human Mediator Subuniti 25. Northeast Structural Genomics Consortium Target HR6188A

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-180759 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mediator of RNA polymerase II transcription subunit 25 Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 18.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q71SY5 (Residues: 391-543; Coverage: 21%)
Gene names: ACID1, ARC92, MED25, PTOV2, TCBAP0758
Sequence domains: Mediator complex subunit 25 PTOV activation and synapsin 2
Structure domains: Mediator complex subunit 25, ACID domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this NMR entry.
Chemical shift assignment: 95%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)