› 2l8l

Solution NMR

Structure of an engineered splicing intein mutant based on Mycobacterium tuberculosis RecA

Released:
DOI: 10.2210/pdb2l8l/pdb
PDB entry 2l8l coloured by chain, ensemble of 20 models, front view.
PDB entry 2l8l coloured by chain, ensemble of 20 models, side view.
PDB entry 2l8l coloured by chain, ensemble of 20 models, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
pK(a) coupling at the intein active site: implications for the coordination mechanism of protein splicing with a conserved aspartate.
Du Z, Zheng Y, Patterson M, Liu Y, Wang C
J Am Chem Soc 133 10275-82 (2011)
PMID: 21604815

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161555 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease PI-MtuI Chain: A
Molecule details ›
Chain: A
Length: 139 amino acids
Theoretical weight: 15.42 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHJ3 (Residues: 252-345, 654-691; Coverage: 17%)
Gene names: MTV002.02c, Rv2737c, recA
Sequence domains: Intein splicing domain
Structure domains: Hedgehog/Intein (Hint) domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this NMR entry.
Chemical shift assignment: 67%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Protein splicing: how inteins escape from precursor proteins.
Mills et al. (2014)
3 more

4 mentions without citation

Structural and dynamical features of inteins and implications on protein splicing.
Eryilmaz et al. (2014)
3 more