› 2las

Solution NMR

Molecular Determinants of Paralogue-Specific SUMO-SIM Recognition

Released:
DOI: 10.2210/pdb2las/pdb
PDB entry 2las coloured by chain, ensemble of 10 models, front view.
PDB entry 2las coloured by chain, ensemble of 10 models, side view.
PDB entry 2las coloured by chain, ensemble of 10 models, top view.
Source organism: Homo sapiens
Primary publication:
Insights into high affinity small ubiquitin-like modifier (SUMO) recognition by SUMO-interacting motifs (SIMs) revealed by a combination of NMR and peptide array analysis.
Namanja AT, Li YJ, Su Y, Wong S, Lu J, Colson LT, Wu C, Li SS, Chen Y
J Biol Chem 287 3231-40 (2012)
PMID: 22147707

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156037 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Small ubiquitin-related modifier 1 Chain: A
Molecule details ›
Chain: A
Length: 101 amino acids
Theoretical weight: 11.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63165 (Residues: 1-101; Coverage: 100%)
Gene names: OK/SW-cl.43, SMT3C, SMT3H3, SUMO1, UBL1
Sequence domains: Ubiquitin-2 like Rad60 SUMO-like
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
E3 SUMO-protein ligase RanBP2 Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.62 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P49792 (Residues: 2705-2717; Coverage: 0%)
Gene names: NUP358, RANBP2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 28%
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

5 review citations

Function and regulation of SUMO proteases.
Hickey et al. (2012)
4 more

5 mentions without citation

Insights into high affinity small ubiquitin-like modifier (SUMO) recognition by SUMO-interacting motifs (SIMs) revealed by a combination of NMR and peptide array analysis.
Namanja et al. (2012)
4 more