› 2ldr

Solution NMR

Solution structure of Helix-RING domain of Cbl-b in the Tyr363 phosphorylated form

Released:
DOI: 10.2210/pdb2ldr/pdb
PDB entry 2ldr coloured by chain, ensemble of 20 models, front view.
PDB entry 2ldr coloured by chain, ensemble of 20 models, side view.
PDB entry 2ldr coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Autoinhibition and phosphorylation-induced activation mechanisms of human cancer and autoimmune disease-related E3 protein Cbl-b.
Kobashigawa Y, Tomitaka A, Kumeta H, Noda NN, Yamaguchi M, Inagaki F
Proc Natl Acad Sci U S A 108 20579-84 (2011)
PMID: 22158902

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171596 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase CBL-B Chain: A
Molecule details ›
Chain: A
Length: 82 amino acids
Theoretical weight: 9.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13191 (Residues: 351-426; Coverage: 8%)
Gene names: CBLB, Nbla00127, RNF56
Sequence domains: Zinc finger, C3HC4 type (RING finger)
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 95%
Refinement method: torsion angle dynamics
Expression system: Escherichia coli

Quick links

Citations

23 review citations

RING-type E3 ligases: master manipulators of E2 ubiquitin-conjugating enzymes and ubiquitination.
Metzger et al. (2014)
22 more

4 mentions without citation

Validation of metal-binding sites in macromolecular structures with the CheckMyMetal web server.
Zheng et al. (2014)
3 more