2le8

Solution NMR

The protein complex for DNA replication

Released:
Source organism: Homo sapiens
Primary publication:
Structural insights into the Cdt1-mediated MCM2-7 chromatin loading.
OpenAccess logo Nucleic Acids Res 40 3208-17 (2012)
PMID: 22140117

Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-172037 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA replication licensing factor MCM6 Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 13.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14566 (Residues: 708-821; Coverage: 14%)
Gene name: MCM6
Sequence domains: MCM6 C-terminal winged-helix domain
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1
DNA replication factor Cdt1 Chain: B
Molecule details ›
Chain: B
Length: 29 amino acids
Theoretical weight: 3.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H211 (Residues: 413-440; Coverage: 5%)
Gene name: CDT1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 59%
Refinement method: torsion angle dynamics
Expression system: Escherichia coli