› 2lec

Solution NMR

Solution structure of human SRSF2 (SC35) RRM in complex with 5'-UGGAGU-3'

Released:
DOI: 10.2210/pdb2lec/pdb
PDB entry 2lec coloured by chain, ensemble of 20 models, front view.
PDB entry 2lec coloured by chain, ensemble of 20 models, side view.
PDB entry 2lec coloured by chain, ensemble of 20 models, top view.
Source organisms:
Primary publication:
A syn-anti conformational difference allows SRSF2 to recognize guanines and cytosines equally well.
Daubner GM, Cléry A, Jayne S, Stevenin J, Allain FH
EMBO J 31 162-74 (2012)
PMID: 22002536

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-554068 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Serine/arginine-rich splicing factor 2 Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 15.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01130 (Residues: 1-101; Coverage: 46%)
Gene names: SFRS2, SRSF2
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
RNA (5'-R(*UP*GP*GP*AP*GP*U)-3') Chain: B
Molecule details ›
Chain: B
Length: 6 nucleotides
Theoretical weight: 1.93 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 68%
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

37 review citations

RNA splicing factors as oncoproteins and tumour suppressors.
Dvinge et al. (2016)
36 more

6 mentions without citation

A deep learning framework to predict binding preference of RNA constituents on protein surface.
Lam et al. (2019)
5 more