› 2ly7

Solution NMR

B-flap domain of RNA polymerase (B. subtilis)

Released:
DOI: 10.2210/pdb2ly7/pdb
PDB entry 2ly7 coloured by chain, ensemble of 20 models, front view.
PDB entry 2ly7 coloured by chain, ensemble of 20 models, side view.
PDB entry 2ly7 coloured by chain, ensemble of 20 models, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
RNA polymerase-induced remodelling of NusA produces a pause enhancement complex.
Ma C, Mobli M, Yang X, Keller AN, King GF, Lewis PJ
OpenAccess logo Nucleic Acids Res 43 2829-40 (2015)
PMID: 25690895

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153515 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerase subunit beta Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.68 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P37870 (Residues: 784-923; Coverage: 12%)
Gene names: BSU01070, crsE, rfm, rpoB
Sequence domains: RNA polymerase Rpb2, domain 6
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 76%
Refinement method: torsion angle dynamics
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Bacterial Transcription as a Target for Antibacterial Drug Development.
Ma et al. (2016)
2 more

3 mentions without citation

Structural biology of bacterial RNA polymerase.
Murakami KS. (2015)
2 more