› 2m0g

Solution NMR

Structure, phosphorylation and U2AF65 binding of the Nterminal Domain of splicing factor 1 during 3 splice site Recognition

Released:
DOI: 10.2210/pdb2m0g/pdb
PDB entry 2m0g coloured by chain, ensemble of 10 models, front view.
PDB entry 2m0g coloured by chain, ensemble of 10 models, side view.
PDB entry 2m0g coloured by chain, ensemble of 10 models, top view.
Source organism: Homo sapiens
Primary publication:
Structure, phosphorylation and U2AF65 binding of the N-terminal domain of splicing factor 1 during 3'-splice site recognition.
Zhang Y, Madl T, Bagdiul I, Kern T, Kang HS, Zou P, Mäusbacher N, Sieber SA, Krämer A, Sattler M
OpenAccess logo Nucleic Acids Res 41 1343-54 (2013)
PMID: 23175611

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150720 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Splicing factor 1 Chain: A
Molecule details ›
Chain: A
Length: 145 amino acids
Theoretical weight: 16.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15637 (Residues: 1-145; Coverage: 23%)
Gene names: SF1, ZFM1, ZNF162
Sequence domains: Splicing factor 1 helix-hairpin domain
Splicing factor U2AF 65 kDa subunit Chain: B
Molecule details ›
Chain: B
Length: 104 amino acids
Theoretical weight: 11.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P26368 (Residues: 372-475; Coverage: 22%)
Gene names: U2AF2, U2AF65
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 34%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

9 review citations

RNA-Binding Proteins: Splicing Factors and Disease.
Fredericks et al. (2015)
8 more

3 mentions without citation

Structural studies of the spliceosome: zooming into the heart of the machine.
Galej et al. (2014)
2 more