› 2m8r

Solution NMR

Pre-Fusion Solution NMR Structure of Neuronal SNARE Syntaxin 1A

Released:
DOI: 10.2210/pdb2m8r/pdb
PDB entry 2m8r coloured by chain, ensemble of 20 models, front view.
PDB entry 2m8r coloured by chain, ensemble of 20 models, side view.
PDB entry 2m8r coloured by chain, ensemble of 20 models, top view.
Source organism: Rattus norvegicus
Primary publication:
Prefusion structure of syntaxin-1A suggests pathway for folding into neuronal trans-SNARE complex fusion intermediate.
Liang B, Kiessling V, Tamm LK
Proc Natl Acad Sci U S A 110 19384-9 (2013)
PMID: 24218570

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-542892 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Syntaxin-1A Chain: A
Molecule details ›
Chain: A
Length: 109 amino acids
Theoretical weight: 12.33 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P32851 (Residues: 183-288; Coverage: 37%)
Gene names: Sap, Stx1a
Sequence domains: SNARE domain
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 91%
Refinement method: distance geometry, simulated annealing
Expression system: Escherichia coli

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Citations

8 review citations

The Multifaceted Role of SNARE Proteins in Membrane Fusion.
Han et al. (2017)
7 more

2 mentions without citation

Solution NMR of SNAREs, complexin and α-synuclein in association with membrane-mimetics.
Liang et al. (2018)
1 more