› 2max

Solution NMR

NMR structure of the RNA polymerase alpha subunit C-terminal domain from Helicobacter pylori

Released:
DOI: 10.2210/pdb2max/pdb
PDB entry 2max coloured by chain, ensemble of 15 models, front view.
PDB entry 2max coloured by chain, ensemble of 15 models, side view.
PDB entry 2max coloured by chain, ensemble of 15 models, top view.
Source organism: Helicobacter pylori J99
Primary publication:
Helicobacter pylori RNA polymerase α-subunit C-terminal domain shows features unique to ɛ-proteobacteria and binds NikR/DNA complexes.
Borin BN, Tang W, Krezel AM
Protein Sci 23 454-63 (2014)
PMID: 24442709

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-579245 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerase subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 126 amino acids
Theoretical weight: 14.16 KDa
Source organism: Helicobacter pylori J99
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZJT5 (Residues: 231-344; Coverage: 33%)
Gene names: jhp_1213, rpoA
Sequence domains: Bacterial RNA polymerase, alpha chain C terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 83%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Insights into the Orchestration of Gene Transcription Regulators in Helicobacter pylori.
Vannini et al. (2022)
1 more

169 mentions without citation

Effects of Exercise Training on Cardiorespiratory Fitness and Biomarkers of Cardiometabolic Health: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.
Lin et al. (2015)
168 more