› 2mch

Solution NMR

Backbone 1H, 13C, and 15N Chemical Shift Assignments for murine norovirus NS1/2 CW3 WT

Released:
DOI: 10.2210/pdb2mch/pdb
PDB entry 2mch coloured by chain, ensemble of 20 models, front view.
PDB entry 2mch coloured by chain, ensemble of 20 models, side view.
PDB entry 2mch coloured by chain, ensemble of 20 models, top view.
Source organism: Murine norovirus 1
Primary publication:
Murine norovirus protein NS1/2 aspartate to glutamate mutation, sufficient for persistence, reorients side chain of surface exposed tryptophan within a novel structured domain.
Borin BN, Tang W, Nice TJ, McCune BT, Virgin HW, Krezel AM
Proteins 82 1200-9 (2014)
PMID: 24273131

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|- and the P1' position is occupied by Gly-|-
NTP + H(2)O = NDP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182353 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NS1 Chain: A
Molecule details ›
Chain: A
Length: 69 amino acids
Theoretical weight: 7.99 KDa
Source organism: Murine norovirus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q80J95 (Residues: 58-114; Coverage: 3%)
Gene name: ORF1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 64%
Refinement method: torsion angle dynamics
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Advances in norovirus biology.
Karst et al. (2014)
7 more

3 mentions without citation

Noroviruses Co-opt the Function of Host Proteins VAPA and VAPB for Replication via a Phenylalanine-Phenylalanine-Acidic-Tract-Motif Mimic in Nonstructural Viral Protein NS1/2.
McCune et al. (2017)
2 more