› 2mx8

Solution NMR

NMR structure of N-terminal domain from A. ventricosus minor ampullate spidroin (MiSp) at pH 7.2

Released:
DOI: 10.2210/pdb2mx8/pdb
PDB entry 2mx8 coloured by chain, ensemble of 20 models, front view.
PDB entry 2mx8 coloured by chain, ensemble of 20 models, side view.
PDB entry 2mx8 coloured by chain, ensemble of 20 models, top view.
Source organism: Araneus ventricosus
Primary publication:
Diversified Structural Basis of a Conserved Molecular Mechanism for pH-Dependent Dimerization in Spider Silk N-Terminal Domains.
Otikovs M, Chen G, Nordling K, Landreh M, Meng Q, Jörnvall H, Kronqvist N, Rising A, Johansson J, Jaudzems K
Chembiochem 16 1720-4 (2015)
PMID: 26033527

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-516517 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Minor ampullate spidroin Chain: A
Molecule details ›
Chain: A
Length: 133 amino acids
Theoretical weight: 14.07 KDa
Source organism: Araneus ventricosus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: K4MTL7 (Residues: 23-151; Coverage: 7%)
Sequence domains: Major ampullate spidroin 1, spider silk protein 1, N-term
Structure domains: Enzyme I; Chain A, domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 93%
Refinement method: simulated annealing, torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Silk Spinning in Silkworms and Spiders.
Andersson et al. (2016)
4 more

2 mentions without citation

The dimerization mechanism of the N-terminal domain of spider silk proteins is conserved despite extensive sequence divergence.
Sarr et al. (2022)
1 more