› 2n21

Solution NMR

Solution structure of complex between DNA G-quadruplex and G-quadruplex recognition domain of RHAU

Released:
DOI: 10.2210/pdb2n21/pdb
PDB entry 2n21 coloured by chain, ensemble of 10 models, front view.
PDB entry 2n21 coloured by chain, ensemble of 10 models, side view.
PDB entry 2n21 coloured by chain, ensemble of 10 models, top view.
Source organisms:
Primary publication:
Insights into G-quadruplex specific recognition by the DEAH-box helicase RHAU: Solution structure of a peptide-quadruplex complex.
Heddi B, Cheong VV, Martadinata H, Phan AT
Proc Natl Acad Sci U S A 112 9608-13 (2015)
PMID: 26195789

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-507034 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
ATP-dependent DNA/RNA helicase DHX36 Chain: A
Molecule details ›
Chain: A
Length: 20 amino acids
Theoretical weight: 2.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H2U1 (Residues: 53-70; Coverage: 2%)
Gene names: DDX36, DHX36, KIAA1488, MLEL1, RHAU
DNA (5'-D(*TP*TP*GP*GP*GP*TP*GP*GP*GP*TP*GP*GP*GP*TP*GP*GP*GP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 18 nucleotides
Theoretical weight: 5.73 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 25%
Refinement method: DGSA-distance geometry simulated annealing, distance geometry, molecular dynamics
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

15 review citations

Replication of G Quadruplex DNA.
Lerner et al. (2019)
14 more

9 mentions without citation

On Characterizing the Interactions between Proteins and Guanine Quadruplex Structures of Nucleic Acids.
McRae et al. (2017)
8 more