› 2n3o

Solution NMR

Structure of PTB RRM1(41-163) bound to an RNA stemloop containing a structured loop derived from viral internal ribosomal entry site RNA

Released:
DOI: 10.2210/pdb2n3o/pdb
PDB entry 2n3o coloured by chain, ensemble of 20 models, front view.
PDB entry 2n3o coloured by chain, ensemble of 20 models, side view.
PDB entry 2n3o coloured by chain, ensemble of 20 models, top view.
Source organisms:
Entry authors: Maris C, Jayne SF, Damberger FF, Ravindranathan S, Allain FH-T

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-541302 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Polypyrimidine tract-binding protein 1 Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 13.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26599 (Residues: 41-163; Coverage: 22%)
Gene names: PTB, PTBP1
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits
RNA (5'-R(*GP*GP*GP*AP*CP*CP*UP*GP*GP*UP*CP*UP*UP*UP*CP*CP*AP*GP*GP*UP*CP*CP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 23 nucleotides
Theoretical weight: 7.31 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 79%
Refinement method: simulated annealing, simulated annealing
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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