› 2o5g

X-ray diffraction
1.08Å resolution

Calmodulin-smooth muscle light chain kinase peptide complex

Released:
DOI: 10.2210/pdb2o5g/pdb
PDB entry 2o5g coloured by chain, front view.
PDB entry 2o5g coloured by chain, side view.
PDB entry 2o5g coloured by chain, top view.
Source organism: Gallus gallus
Entry authors: Valentine KG, Ng HL, Schneeweis JK, Kranz JK, Frederick KK, Alber T, Wand AJ

Function and Biology Details

Reaction catalysed: 
ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-536607 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.72 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62149 (Residues: 2-149; Coverage: 99%)
Gene names: CALM, CAM, RCJMB04_24e7
Sequence domains: EF-hand domain pair
Structure domains: EF-hand
Myosin light chain kinase, smooth muscle, deglutamylated form Chain: B
Molecule details ›
Chain: B
Length: 21 amino acids
Theoretical weight: 2.22 KDa
Source organism: Gallus gallus
Expression system: Not provided
UniProt:
  • Canonical: P11799 (Residues: 1730-1748; Coverage: 1%)
Gene name: Mylk

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 28.861Å b: 56.894Å c: 44.76Å
α: 90° β: 97.45° γ: 90°
R-values:
R R work R free
0.144 0.142 0.165
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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