› 2oaw

X-ray diffraction
1.9Å resolution

Structure of SHH variant of "Bergerac" chimera of spectrin SH3

Released:
DOI: 10.2210/pdb2oaw/pdb
PDB entry 2oaw coloured by chain, front view.
PDB entry 2oaw coloured by chain, side view.
PDB entry 2oaw coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Structural and thermodynamic studies of Bergerac-SH3 chimeras.
Gushchina LV, Gabdulkhakov AG, Nikonov SV, Mateo PL, Filimonov VV
Biophys Chem 139 106-15 (2009)
PMID: 19042078

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-139741 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spectrin alpha chain, non-erythrocytic 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 65 amino acids
Theoretical weight: 7.6 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07751 (Residues: 969-1009, 1010-1011, 1012-1025; Coverage: 2%)
Gene names: SPTA2, SPTAN1
Sequence domains: SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

1 bound ligand:
Ligand CL 9 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: P212121
Unit cell:
a: 49.47Å b: 57.55Å c: 101.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

High-resolution crystal structure of spectrin SH3 domain fused with a proline-rich peptide.
Gushchina et al. (2011)
1 more

1 mention without citation

Apparent structural differences at the tetramerization region of erythroid and nonerythroid beta spectrin as discriminated by phage displayed scFvs.
Song et al. (2011)