› 2orj

X-ray diffraction
1.8Å resolution

crystal structure of the trimeric neck and carbohydrate recognition domain of human surfactant protein D in complex with N-acetyl mannosamine

Released:
DOI: 10.2210/pdb2orj/pdb
PDB entry 2orj coloured by chain, front view.
PDB entry 2orj coloured by chain, side view.
PDB entry 2orj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Critical role of Arg/Lys343 in the species-dependent recognition of phosphatidylinositol by pulmonary surfactant protein D.
Crouch E, McDonald B, Smith K, Roberts M, Mealy T, Seaton B, Head J
Biochemistry 46 5160-9 (2007)
PMID: 17417879

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152870 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pulmonary surfactant-associated protein D Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 160 amino acids
Theoretical weight: 17.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35247 (Residues: 223-375; Coverage: 43%)
Gene names: COLEC7, PSPD, SFTP4, SFTPD
Sequence domains:
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

2 bound ligands:
Ligand CA 9 x CA
Ligand BM3 3 x BM3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 55.63Å b: 108.33Å c: 55.97Å
α: 90° β: 91.51° γ: 90°
R-values:
R R work R free
0.181 0.178 0.205
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structure-function relationships in pulmonary surfactant membranes: from biophysics to therapy.
Lopez-Rodriguez et al. (2014)
3 more

4 mentions without citation

Structural features, evolutionary relationships, and transcriptional regulation of C-type lectin-domain proteins in Manduca sexta.
Rao et al. (2015)
3 more