› 2osl

X-ray diffraction
2.6Å resolution

Crystal structure of Rituximab Fab in complex with an epitope peptide

Released:
DOI: 10.2210/pdb2osl/pdb
PDB entry 2osl coloured by chain, front view.
PDB entry 2osl coloured by chain, side view.
PDB entry 2osl coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for recognition of CD20 by therapeutic antibody Rituximab.
Du J, Wang H, Zhong C, Peng B, Zhang M, Li B, Huo S, Guo Y, Ding J
J Biol Chem 282 15073-80 (2007)
PMID: 17395584

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-233281 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
light chain of the Rituximab Fab fragment,light chain of the Rituximab Fab fragment Chains: B, L
Molecule details ›
Chains: B, L
Length: 213 amino acids
Theoretical weight: 23.08 KDa
Source organism: Mus musculus
Expression system: synthetic construct
Structure domains: Immunoglobulins
heavy chain of the Rituximab Fab fragment,heavy chain of the Rituximab Fab fragment Chains: A, H
Molecule details ›
Chains: A, H
Length: 224 amino acids
Theoretical weight: 23.73 KDa
Source organism: Mus musculus
Expression system: synthetic construct
Structure domains: Immunoglobulins
B-lymphocyte antigen CD20 Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 25 amino acids
Theoretical weight: 2.85 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P11836 (Residues: 163-187; Coverage: 8%)
Gene names: CD20, MS4A1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 96.421Å b: 98.809Å c: 107.241Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.239 0.296
Expression systems:
  • synthetic construct
  • Not provided

Quick links

Citations

14 review citations

A review of the current use of rituximab in autoimmune diseases.
Gürcan et al. (2009)
13 more

11 mentions without citation

Antibody drug conjugates: design and selection of linker, payload and conjugation chemistry.
McCombs et al. (2015)
10 more