› 2p9p

X-ray diffraction
2.9Å resolution

Crystal Structure of bovine Arp2/3 complex co-crystallized with ADP

Released:
DOI: 10.2210/pdb2p9p/pdb
PDB entry 2p9p coloured by chain, front view.
PDB entry 2p9p coloured by chain, side view.
PDB entry 2p9p coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Insights into the influence of nucleotides on actin family proteins from seven structures of Arp2/3 complex.
Nolen BJ, Pollard TD
Mol Cell 26 449-57 (2007)
PMID: 17499050

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-501200 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Actin-related protein 3 Chain: A
Molecule details ›
Chain: A
Length: 418 amino acids
Theoretical weight: 47.43 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P61157 (Residues: 1-418; Coverage: 100%)
Gene names: ACTR3, ARP3
Sequence domains: Actin
Structure domains:
Actin-related protein 2 Chain: B
Molecule details ›
Chain: B
Length: 394 amino acids
Theoretical weight: 44.82 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: A7MB62 (Residues: 1-394; Coverage: 100%)
Gene names: ACTR2, ARP2
Sequence domains: Actin
Structure domains:
Actin-related protein 2/3 complex subunit 1B Chain: C
Molecule details ›
Chain: C
Length: 372 amino acids
Theoretical weight: 41.02 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: Q58CQ2 (Residues: 1-372; Coverage: 100%)
Gene name: ARPC1B
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Actin-related protein 2/3 complex subunit 2 Chain: D
Molecule details ›
Chain: D
Length: 300 amino acids
Theoretical weight: 34.4 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: Q3MHR7 (Residues: 1-300; Coverage: 100%)
Gene name: ARPC2
Sequence domains: Arp2/3 complex, 34 kD subunit p34-Arc
Structure domains: Yope Regulator; Chain: A,
Actin-related protein 2/3 complex subunit 3 Chain: E
Molecule details ›
Chain: E
Length: 178 amino acids
Theoretical weight: 20.57 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: Q3T035 (Residues: 1-178; Coverage: 100%)
Gene name: ARPC3
Sequence domains: ARP2/3 complex ARPC3 (21 kDa) subunit
Structure domains: Actin-related protein 2/3 complex subunit 3
Actin-related protein 2/3 complex subunit 4 Chain: F
Molecule details ›
Chain: F
Length: 168 amino acids
Theoretical weight: 19.7 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: Q148J6 (Residues: 1-168; Coverage: 100%)
Gene name: ARPC4
Sequence domains: ARP2/3 complex 20 kDa subunit (ARPC4)
Structure domains: Yope Regulator; Chain: A,
Actin-related protein 2/3 complex subunit 5 Chain: G
Molecule details ›
Chain: G
Length: 151 amino acids
Theoretical weight: 16.3 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: Q3SYX9 (Residues: 1-151; Coverage: 100%)
Gene name: ARPC5
Sequence domains: ARP2/3 complex 16 kDa subunit (p16-Arc)
Structure domains: Actin-related protein 2/3 complex subunit 5

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 110.966Å b: 128.926Å c: 202.288Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.234 0.288
Expression system: Not provided

Quick links

Citations

11 review citations

Regulation of actin filament assembly by Arp2/3 complex and formins.
Pollard TD. (2007)
10 more

5 mentions without citation

UCSF Chimera, MODELLER, and IMP: an integrated modeling system.
Yang et al. (2012)
4 more