› 2rl9

X-ray diffraction
2.4Å resolution

Crystal Structure cation-dependent mannose 6-phosphate receptor at pH 6.5 bound to trimannoside

Released:
DOI: 10.2210/pdb2rl9/pdb
PDB entry 2rl9 coloured by chain, front view.
PDB entry 2rl9 coloured by chain, side view.
PDB entry 2rl9 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structural insights into the mechanism of pH-dependent ligand binding and release by the cation-dependent mannose 6-phosphate receptor.
Olson LJ, Hindsgaul O, Dahms NM, Kim JJ
J Biol Chem 283 10124-34 (2008)
PMID: 18272523

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145846 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Cation-dependent mannose-6-phosphate receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 154 amino acids
Theoretical weight: 17.44 KDa
Source organism: Bos taurus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P11456 (Residues: 29-182; Coverage: 61%)
Gene name: M6PR
Sequence domains: Mannose-6-phosphate receptor
Structure domains: Mannose-6-phosphate receptor binding domain

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN, M6P
Carbohydrate polymer
2 bound ligands:
Ligand NAG 2 x NAG
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 92.628Å b: 92.628Å c: 85.594Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.265
Expression system: Trichoplusia ni

Quick links

Citations

8 review citations

The repertoire of glycan determinants in the human glycome.
Cummings RD. (2009)
7 more

1 mention without citation

Structural insights into the mechanism of pH-dependent ligand binding and release by the cation-dependent mannose 6-phosphate receptor.
Olson et al. (2008)