› 2vk2

X-ray diffraction
1.2Å resolution

Crystal structure of a galactofuranose binding protein

Released:
DOI: 10.2210/pdb2vk2/pdb
PDB entry 2vk2 coloured by chain, front view.
PDB entry 2vk2 coloured by chain, side view.
PDB entry 2vk2 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Furanose-specific sugar transport: characterization of a bacterial galactofuranose-binding protein.
Horler RS, Müller A, Williamson DC, Potts JR, Wilson KS, Thomas GH
J Biol Chem 284 31156-63 (2009)
PMID: 19744923

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153866 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactofuranose-binding protein YtfQ Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 33.38 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P39325 (Residues: 22-318; Coverage: 100%)
Gene names: JW4186, b4227, ytfQ
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand GZL 1 x GZL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 45.745Å b: 51.535Å c: 116.091Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.178 0.19
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 citation in other articles

Genome-scale analyses of Escherichia coli and Salmonella enterica AraC reveal noncanonical targets and an expanded core regulon.
Stringer et al. (2014)
12 more

5 mentions without citation

Molecular insights on the recognition of a Lactococcus lactis cell wall pellicle by the phage 1358 receptor binding protein.
Farenc et al. (2014)
4 more