› 2vnf

X-ray diffraction
1.76Å resolution

MOLECULAR BASIS OF HISTONE H3K4ME3 RECOGNITION BY ING4

Released:
DOI: 10.2210/pdb2vnf/pdb
PDB entry 2vnf coloured by chain, front view.
PDB entry 2vnf coloured by chain, side view.
PDB entry 2vnf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis of histone H3K4me3 recognition by ING4.
Palacios A, Muñoz IG, Pantoja-Uceda D, Marcaida MJ, Torres D, Martín-García JM, Luque I, Montoya G, Blanco FJ
J Biol Chem 283 15956-64 (2008)
PMID: 18381289

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-549971 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Inhibitor of growth protein 4 Chains: A, C
Molecule details ›
Chains: A, C
Length: 60 amino acids
Theoretical weight: 6.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UNL4 (Residues: 188-246; Coverage: 24%)
Gene names: ING4, My036
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)
Histone H3.1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 10 amino acids
Theoretical weight: 1.19 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 2-11; Coverage: 7%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

4 bound ligands:
Ligand NA 1 x NA
Ligand DTT 1 x DTT
Ligand ZN 4 x ZN
Ligand DTU 1 x DTU
1 modified residue:
Ligand M3L 2 x M3L

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43
Unit cell:
a: 68.516Å b: 68.516Å c: 28.174Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.156 0.227
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

17 review citations

PHD fingers in human diseases: disorders arising from misinterpreting epigenetic marks.
Baker et al. (2008)
16 more

6 mentions without citation

PHD fingers: epigenetic effectors and potential drug targets.
Musselman et al. (2009)
5 more