› 2w0f

X-ray diffraction
2.4Å resolution

Potassium Channel KcsA-Fab Complex with Tetraoctylammonium

Released:
DOI: 10.2210/pdb2w0f/pdb
PDB entry 2w0f coloured by chain, front view.
PDB entry 2w0f coloured by chain, side view.
PDB entry 2w0f coloured by chain, top view.
Source organisms:
Primary publication:
Structures of KcsA in complex with symmetrical quaternary ammonium compounds reveal a hydrophobic binding site.
Lenaeus MJ, Burdette D, Wagner T, Focia PJ, Gross A
OpenAccess logo Biochemistry 53 5365-73 (2014)
PMID: 25093676

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209463 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ANTIBODY FAB FRAGMENT LIGHT CHAIN Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.41 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
ANTIBODY FAB FRAGMENT HEAVY CHAIN Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.44 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
pH-gated potassium channel KcsA Chain: C
Molecule details ›
Chain: C
Length: 124 amino acids
Theoretical weight: 13.24 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A334 (Residues: 1-124; Coverage: 78%)
Gene names: kcsA, skc1
Sequence domains:
Structure domains: Helix Hairpins

Ligands and Environments

5 bound ligands:
Ligand CO 1 x CO
Ligand F09 1 x F09
Ligand DGA 1 x DGA
Ligand HX0 1 x HX0
Ligand K 7 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I4
Unit cell:
a: 155.76Å b: 155.76Å c: 75.889Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.2 0.241
Expression systems:
  • Mus musculus
  • Escherichia coli

Quick links

Citations

3 review citations

Membrane Protein Structure, Function, and Dynamics: a Perspective from Experiments and Theory.
Cournia et al. (2015)
2 more

4 mentions without citation

Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance.
Enkavi et al. (2019)
3 more