› 2w5o

X-ray diffraction
2.05Å resolution

Complex structure of the GH93 alpha-L-arabinofuranosidase of Fusarium graminearum with arabinobiose

Released:
DOI: 10.2210/pdb2w5o/pdb
PDB entry 2w5o coloured by chain, front view.
PDB entry 2w5o coloured by chain, side view.
PDB entry 2w5o coloured by chain, top view.
Source organism: Fusarium graminearum
Primary publication:
Molecular basis of arabinobio-hydrolase activity in phytopathogenic fungi: crystal structure and catalytic mechanism of Fusarium graminearum GH93 exo-alpha-L-arabinanase.
Carapito R, Imberty A, Jeltsch JM, Byrns SC, Tam PH, Lowary TL, Varrot A, Phalip V
J Biol Chem 284 12285-96 (2009)
PMID: 19269961

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163285 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
ALPHA-L-ARABINOFURANOSIDASE Chain: A
Molecule details ›
Chain: A
Length: 367 amino acids
Theoretical weight: 41.4 KDa
Source organism: Fusarium graminearum
Expression system: Escherichia coli BL21(DE3)
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: AHR
Carbohydrate polymer
3 bound ligands:
Ligand EDO 5 x EDO
Ligand SO4 1 x SO4
Ligand PEG 1 x PEG
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 48.62Å b: 86.88Å c: 108.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.168 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Fungal arabinan and L-arabinose metabolism.
Seiboth et al. (2011)
6 more

1 mention without citation

Molecular basis of arabinobio-hydrolase activity in phytopathogenic fungi: crystal structure and catalytic mechanism of Fusarium graminearum GH93 exo-alpha-L-arabinanase.
Carapito et al. (2009)