› 2xdf

Hybrid

Solution Structure of the Enzyme I Dimer Complexed with HPr Using Residual Dipolar Couplings and Small Angle X-Ray Scattering

Released:
DOI: 10.2210/pdb2xdf/pdb
PDB entry 2xdf coloured by chain, ensemble of 2 models, front view.
PDB entry 2xdf coloured by chain, ensemble of 2 models, side view.
PDB entry 2xdf coloured by chain, ensemble of 2 models, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
Solution structure of the 128 kDa enzyme I dimer from Escherichia coli and its 146 kDa complex with HPr using residual dipolar couplings and small- and wide-angle X-ray scattering.
Schwieters CD, Suh JY, Grishaev A, Ghirlando R, Takayama Y, Clore GM
J Am Chem Soc 132 13026-45 (2010)
PMID: 20731394

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140396 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phosphoenolpyruvate-protein phosphotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 573 amino acids
Theoretical weight: 63.42 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08839 (Residues: 1-573; Coverage: 100%)
Gene names: JW2409, b2416, ptsI
Sequence domains:
Structure domains: Phosphoenolpyruvate-binding domains
Phosphocarrier protein HPr Chains: C, D
Molecule details ›
Chains: C, D
Length: 85 amino acids
Theoretical weight: 9.13 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AA04 (Residues: 1-85; Coverage: 100%)
Gene names: JW2408, b2415, hpr, ptsH
Sequence domains: PTS HPr component phosphorylation site
Structure domains: HPr-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Experimental Method: X-ray solution scattering, Solution NMR
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

A practical guide to small angle X-ray scattering (SAXS) of flexible and intrinsically disordered proteins.
Kikhney et al. (2015)
12 more

2 mentions without citation

Using small angle solution scattering data in Xplor-NIH structure calculations.
Schwieters et al. (2014)
1 more