› 2xxa

X-ray diffraction
3.94Å resolution

The Crystal Structure of the Signal Recognition Particle (SRP) in Complex with its Receptor(SR)

Released:
DOI: 10.2210/pdb2xxa/pdb
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PDB entry 2xxa coloured by chain, front view.
PDB entry 2xxa coloured by chain, side view.
PDB entry 2xxa coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The crystal structure of the signal recognition particle in complex with its receptor.
Ataide SF, Schmitz N, Shen K, Ke A, Shan SO, Doudna JA, Ban N
Science 331 881-6 (2011)
PMID: 21330537

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-287524 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Signal recognition particle protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 433 amino acids
Theoretical weight: 47.66 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AGD7 (Residues: 1-433; Coverage: 96%)
Gene names: JW5414, b2610, ffh
Sequence domains:
Structure domains:
Signal recognition particle receptor FtsY Chains: B, D
Molecule details ›
Chains: B, D
Length: 302 amino acids
Theoretical weight: 33.04 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10121 (Residues: 196-497; Coverage: 61%)
Gene names: JW3429, b3464, ftsY
Sequence domains:
Structure domains:
4.5S RNA Chains: F, G

Ligands and Environments

2 bound ligands:
Ligand GCP 4 x GCP
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 84.1Å b: 131.04Å c: 266.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.232 0.267
Expression system: Escherichia coli BL21(DE3)

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Citations

24 review citations

Mechanisms of integral membrane protein insertion and folding.
Cymer et al. (2015)
23 more

22 mentions without citation

Signal recognition particle: an essential protein-targeting machine.
Akopian et al. (2013)
21 more

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