› 2ynt

X-ray diffraction
1.6Å resolution

GIM-1-3Mol native. Crystal structures of Pseudomonas aeruginosa GIM- 1: active site plasticity in metallo-beta-lactamases

Released:
DOI: 10.2210/pdb2ynt/pdb
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PDB entry 2ynt coloured by chain, front view.
PDB entry 2ynt coloured by chain, side view.
PDB entry 2ynt coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal structures of Pseudomonas aeruginosa GIM-1: active-site plasticity in metallo-β-lactamases.
Borra PS, Samuelsen Ø, Spencer J, Walsh TR, Lorentzen MS, Leiros HK
Antimicrob Agents Chemother 57 848-54 (2013)
PMID: 23208706

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180638 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Metallo-beta-lactamase domain-containing protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 233 amino acids
Theoretical weight: 25.62 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q704V1 (Residues: 19-250; Coverage: 100%)
Gene names: blaGIM-1, blaGim-1
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like
Metallo-beta-lactamase domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 233 amino acids
Theoretical weight: 25.63 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q704V1 (Residues: 19-250; Coverage: 100%)
Gene names: blaGIM-1, blaGim-1
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

2 bound ligands:
Ligand ZN 6 x ZN
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21212
Unit cell:
a: 110.922Å b: 140.658Å c: 41.069Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.205
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Structural basis for carbapenem-hydrolyzing mechanisms of carbapenemases conferring antibiotic resistance.
Jeon et al. (2015)
2 more

6 mentions without citation

Extensive mutagenesis experiments corroborate a structural model for the DNA deaminase domain of APOBEC3G.
Chen et al. (2007)
5 more

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