› 2z33

Solution NMR

Solution structure of the DNA complex of PhoB DNA-binding/transactivation Domain

Released:
DOI: 10.2210/pdb2z33/pdb
PDB entry 2z33 coloured by chain, ensemble of 10 models, front view.
PDB entry 2z33 coloured by chain, ensemble of 10 models, side view.
PDB entry 2z33 coloured by chain, ensemble of 10 models, top view.
Source organism: Escherichia coli
Primary publication:
Water-mediated interactions between DNA and PhoB DNA-binding/transactivation domain: NMR-restrained molecular dynamics in explicit water environment.
Yamane T, Okamura H, Ikeguchi M, Nishimura Y, Kidera A
Proteins 71 1970-83 (2008)
PMID: 18186481

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115958 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Phosphate regulon transcriptional regulatory protein PhoB Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 12.17 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFJ5 (Residues: 126-229; Coverage: 45%)
Gene names: JW0389, b0399, phoB
Sequence domains: Transcriptional regulatory protein, C terminal
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(*AP*CP*TP*GP*TP*CP*AP*TP*AP*AP*AP*TP*CP*TP*GP*T)-3' Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.87 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*AP*CP*AP*GP*AP*TP*TP*TP*AP*TP*GP*AP*CP*AP*GP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.92 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing molecluar dynamics
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Global regulation by the seven-component Pi signaling system.
Hsieh et al. (2010)
5 more

3 mentions without citation

The replication initiator of the cholera pathogen's second chromosome shows structural similarity to plasmid initiators.
Orlova et al. (2017)
2 more