› 2a1k

X-ray diffraction
2Å resolution

RB69 single-stranded DNA binding protein core domain

Released:
DOI: 10.2210/pdb2a1k/pdb
PDB entry 2a1k coloured by chain, front view.
PDB entry 2a1k coloured by chain, side view.
PDB entry 2a1k coloured by chain, top view.
Source organism: Escherichia phage RB69
Primary publication:
Structure and enzymatic properties of a chimeric bacteriophage RB69 DNA polymerase and single-stranded DNA binding protein with increased processivity.
Sun S, Geng L, Shamoo Y
Proteins 65 231-8 (2006)
PMID: 16881051

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182201 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Single-stranded DNA-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 233 amino acids
Theoretical weight: 26.1 KDa
Source organism: Escherichia phage RB69
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7Y265 (Residues: 21-253; Coverage: 78%)
Gene name: 32
Sequence domains: gp32 DNA binding protein like
Structure domains: Replication Fork Single-Stranded Dna Binding Protein

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P43
Unit cell:
a: 67.7Å b: 67.7Å c: 123.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.236 0.259
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives.
Mueser et al. (2010)
1 more

3 mentions without citation

Disulfide conformation and design at helix N-termini.
Indu et al. (2010)
2 more