› 2a29

Solution NMR

The solution structure of the AMP-PNP bound nucleotide binding domain of KdpB

Released:
DOI: 10.2210/pdb2a29/pdb
PDB entry 2a29 coloured by chain, front view.
PDB entry 2a29 coloured by chain, side view.
PDB entry 2a29 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The holo-form of the nucleotide binding domain of the KdpFABC complex from Escherichia coli reveals a new binding mode.
Haupt M, Bramkamp M, Heller M, Coles M, Deckers-Hebestreit G, Herkenhoff-Hesselmann B, Altendorf K, Kessler H
J Biol Chem 281 9641-9 (2006)
PMID: 16354672

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + K(+)(Side 1) = ADP + phosphate + K(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137302 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium-transporting ATPase ATP-binding subunit Chain: A
Molecule details ›
Chain: A
Length: 156 amino acids
Theoretical weight: 17.17 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03960 (Residues: 316-451; Coverage: 20%)
Gene names: JW0685, b0697, kdpB
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains: Calcium-transporting ATPase, cytoplasmic domain N

Ligands and Environments

1 bound ligand:
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this NMR entry.
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

P-type ATPases.
Palmgren et al. (2011)
4 more