2a7e Citations

On the routine use of soft X-rays in macromolecular crystallography. Part III. The optimal data-collection wavelength.

Acta Crystallogr D Biol Crystallogr 61 1263-72 (2005)
Related entries: 2a7a, 2a7b, 2a7c, 2a7d, 2a7f, 2a7g, 2a7h, 2a7i, 2a7j

Cited: 23 times
EuropePMC logo PMID: 16131760

Abstract

Complete and highly redundant data sets were collected at different wavelengths between 0.80 and 2.65 A for a total of ten different protein and DNA model systems. The magnitude of the anomalous signal-to-noise ratio as assessed by the quotient R(anom)/R(r.i.m.) was found to be influenced by the data-collection wavelength and the nature of the anomalously scattering substructure. By utilizing simple empirical correlations, for instance between the estimated deltaF/F and the expected R(anom) or the data-collection wavelength and the expected R(r.i.m.), the wavelength at which the highest anomalous signal-to-noise ratio can be expected could be estimated even before the experiment. Almost independent of the nature of the anomalously scattering substructure and provided that no elemental X-ray absorption edge is nearby, this optimal wavelength is 2.1 A.

Articles - 2a7e mentioned but not cited (1)



Reviews citing this publication (2)

  1. SAD phasing: History, current impact and future opportunities. Rose JP, Wang BC. Arch Biochem Biophys 602 80-94 (2016)
  2. Collection of X-Ray Diffraction Data from Macromolecular Crystals. Dauter Z. Methods Mol Biol 1607 165-184 (2017)

Articles citing this publication (20)