› 2aaj

X-ray diffraction
2.74Å resolution

Crystal Structures of the Wild-type, Mutant-P1A and Inactivated Malonate Semialdehyde Decarboxylase: A Structural Basis for the Decarboxylase and Hydratase Activities

Released:
DOI: 10.2210/pdb2aaj/pdb
PDB entry 2aaj coloured by chain, front view.
PDB entry 2aaj coloured by chain, side view.
PDB entry 2aaj coloured by chain, top view.
Source organism: Pseudomonas pavonaceae
Primary publication:
Crystal structures of the wild-type, P1A mutant, and inactivated malonate semialdehyde decarboxylase: a structural basis for the decarboxylase and hydratase activities.
Almrud JJ, Poelarends GJ, Johnson WH, Serrano H, Hackert ML, Whitman CP
Biochemistry 44 14818-27 (2005)
PMID: 16274229

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190201 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malonate semialdehyde decarboxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 130 amino acids
Theoretical weight: 14.11 KDa
Source organism: Pseudomonas pavonaceae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9EV83 (Residues: 2-130; Coverage: 99%)
Gene name: msaD
Sequence domains: Tautomerase enzyme
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P213
Unit cell:
a: 110.152Å b: 110.152Å c: 110.152Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.161 0.213
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The chemical versatility of the beta-alpha-beta fold: catalytic promiscuity and divergent evolution in the tautomerase superfamily.
Poelarends et al. (2008)
1 more

1 mention without citation

Kinetic, mutational, and structural analysis of malonate semialdehyde decarboxylase from Coryneform bacterium strain FG41: mechanistic implications for the decarboxylase and hydratase activities.
Guo et al. (2013)