› 2acx

X-ray diffraction
2.6Å resolution

Crystal Structure of G protein coupled receptor kinase 6 bound to AMPPNP

Released:
DOI: 10.2210/pdb2acx/pdb
PDB entry 2acx coloured by chain, front view.
PDB entry 2acx coloured by chain, side view.
PDB entry 2acx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of G protein-coupled receptor kinase (GRK)-6 defines a second lineage of GRKs.
Lodowski DT, Tesmer VM, Benovic JL, Tesmer JJ
J Biol Chem 281 16785-93 (2006)
PMID: 16613860

Function and Biology Details

Reaction catalysed: 
ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155005 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
G protein-coupled receptor kinase 6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 576 amino acids
Theoretical weight: 66.01 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P43250 (Residues: 1-576; Coverage: 100%)
  • Best match: P43250-2 (Residues: 1-559)
Gene names: GPRK6, GRK6
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand PO4 2 x PO4
Ligand ANP 2 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 120.164Å b: 59.27Å c: 221.095Å
α: 90° β: 102.58° γ: 90°
R-values:
R R work R free
0.204 0.202 0.243
Expression system: Trichoplusia ni

Quick links

Citations

17 review citations

G protein-coupled receptor kinases: more than just kinases and not only for GPCRs.
Gurevich et al. (2012)
16 more

14 mentions without citation

Discovery of new GPCR biology: one receptor structure at a time.
Hanson et al. (2009)
13 more