› 2akc

X-ray diffraction
2.3Å resolution

Crystal structure of tungstate complex of the PhoN protein from S. typhimurium

Released:
DOI: 10.2210/pdb2akc/pdb
PDB entry 2akc coloured by chain, front view.
PDB entry 2akc coloured by chain, side view.
PDB entry 2akc coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
Structure and mutational analysis of the PhoN protein of Salmonella typhimurium provide insight into mechanistic details.
Makde RD, Mahajan SK, Kumar V
Biochemistry 46 2079-90 (2007)
PMID: 17263560

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185212 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acid phosphatase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 230 amino acids
Theoretical weight: 26.09 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8KRU6 (Residues: 21-250; Coverage: 100%)
Gene name: phoN
Sequence domains: PAP2 superfamily
Structure domains: Phosphatidic acid phosphatase type 2/haloperoxidase

Ligands and Environments

1 bound ligand:
Ligand WO4 4 x WO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 189.8Å b: 45.2Å c: 112.7Å
α: 90° β: 111.4° γ: 90°
R-values:
R R work R free
0.164 0.164 0.211
Expression system: Escherichia coli

Quick links

Citations

10 citation in other articles

Proposed carrier lipid-binding site of undecaprenyl pyrophosphate phosphatase from Escherichia coli.
Chang et al. (2014)
9 more

3 mentions without citation

The catalytic scaffold of the haloalkanoic acid dehalogenase enzyme superfamily acts as a mold for the trigonal bipyramidal transition state.
Lu et al. (2008)
2 more