› 2ale

X-ray diffraction
1.8Å resolution

Crystal structure of yeast RNA splicing factor Snu13p

Released:
DOI: 10.2210/pdb2ale/pdb
PDB entry 2ale coloured by chain, front view.
PDB entry 2ale coloured by chain, side view.
PDB entry 2ale coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Analysis of pre-mRNA and pre-rRNA processing factor Snu13p structure and mutants.
Dobbyn HC, McEwan PA, Krause A, Novak-Frazer L, Bella J, O'Keefe RT
Biochem Biophys Res Commun 360 857-62 (2007)
PMID: 17631273

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-544373 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
13 kDa ribonucleoprotein-associated protein Chain: A
Molecule details ›
Chain: A
Length: 134 amino acids
Theoretical weight: 14.65 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P39990 (Residues: 1-126; Coverage: 100%)
Gene names: SNU13, YEL026W
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Structure domains: 60s Ribosomal Protein L30; Chain: A;

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P3121
Unit cell:
a: 70.33Å b: 70.33Å c: 53.303Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.251 0.223 0.248
Expression system: Escherichia coli BL21

Quick links

Citations

1 citations in other articles

The 3.8 Å structure of the U4/U6.U5 tri-snRNP: Insights into spliceosome assembly and catalysis.
Wan et al. (2016)

8 mentions without citation

The architecture of the spliceosomal U4/U6.U5 tri-snRNP.
Nguyen et al. (2015)
7 more