› 2amj

X-ray diffraction
1.8Å resolution

Crystal Structure of Modulator of Drug Activity B from Escherichia coli O157:H7

Released:
DOI: 10.2210/pdb2amj/pdb
PDB entry 2amj coloured by chain, front view.
PDB entry 2amj coloured by chain, side view.
PDB entry 2amj coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
Modulator of drug activity B from Escherichia coli: crystal structure of a prokaryotic homologue of DT-diaphorase.
Adams MA, Jia Z
J Mol Biol 359 455-65 (2006)
PMID: 16630630

Function and Biology Details

Reaction catalysed: 
NADPH + a quinone = NADP(+) + a quinol
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142670 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH:quinone oxidoreductase MdaB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 204 amino acids
Theoretical weight: 23.45 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEY7 (Residues: 2-193; Coverage: 100%)
Gene names: ECs3910, Z4379, mdaB
Sequence domains: Flavodoxin-like fold
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P21
Unit cell:
a: 53.056Å b: 86.51Å c: 82.667Å
α: 90° β: 93.85° γ: 90°
R-values:
R R work R free
0.233 0.233 0.263
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

The diverse antioxidant systems of Helicobacter pylori.
Wang et al. (2006)
4 more

3 mentions without citation

Identifying the subproteome of kinetically stable proteins via diagonal 2D SDS/PAGE.
Xia et al. (2007)
2 more