› 2aq4

X-ray diffraction
2.32Å resolution

Ternary complex of the catalytic core of REV1 with DNA and dCTP.

Released:
DOI: 10.2210/pdb2aq4/pdb
PDB entry 2aq4 coloured by chain, front view.
PDB entry 2aq4 coloured by chain, side view.
PDB entry 2aq4 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Rev1 employs a novel mechanism of DNA synthesis using a protein template.
Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK
Science 309 2219-22 (2005)
PMID: 16195463

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115080 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA repair protein REV1 Chain: A
Molecule details ›
Chain: A
Length: 434 amino acids
Theoretical weight: 49.45 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P12689 (Residues: 305-738; Coverage: 44%)
Gene names: O6339, REV1, YOR346W
Sequence domains:
Structure domains:
5'-D(*AP*TP*CP*CP*TP*CP*CP*CP*CP*TP*AP*C)-3' Chain: P
Molecule details ›
Chain: P
Length: 12 nucleotides
Theoretical weight: 3.5 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
5'-D(*TP*AP*AP*GP*GP*TP*AP*GP*GP*GP*GP*AP*GP*GP*AP*T)-3' Chain: T
Molecule details ›
Chain: T
Length: 16 nucleotides
Theoretical weight: 5.07 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand DCP 1 x DCP
1 modified residue:
Ligand DOC 1 x DOC

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A, NSLS BEAMLINE X4A
Spacegroup: C2221
Unit cell:
a: 177.32Å b: 200.906Å c: 55.586Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.238
Expression systems:
  • Saccharomyces cerevisiae
  • Not provided

Quick links

Citations

40 review citations

Y-family DNA polymerases and their role in tolerance of cellular DNA damage.
Sale et al. (2012)
39 more

8 mentions without citation

An overview of Y-Family DNA polymerases and a case study of human DNA polymerase η.
Yang W. (2014)
7 more