› 2aqx

X-ray diffraction
2.5Å resolution

Crystal Structure of the Catalytic and CaM-Binding domains of Inositol 1,4,5-Trisphosphate 3-Kinase B

Released:
DOI: 10.2210/pdb2aqx/pdb
PDB entry 2aqx coloured by chain, front view.
PDB entry 2aqx coloured by chain, side view.
PDB entry 2aqx coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural insights into enzyme regulation for inositol 1,4,5-trisphosphate 3-kinase B.
Chamberlain PP, Sandberg ML, Sauer K, Cooke MP, Lesley SA, Spraggon G
Biochemistry 44 14486-93 (2005)
PMID: 16262249

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109718 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 289 amino acids
Theoretical weight: 33.43 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: B2RXC2 (Residues: 650-938; Coverage: 31%)
Gene name: Itpkb
Sequence domains: Inositol polyphosphate kinase
Structure domains: Inositol polyphosphate kinase

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P1
Unit cell:
a: 53.204Å b: 60.655Å c: 56.851Å
α: 59.9° β: 72.74° γ: 88.18°
R-values:
R R work R free
0.189 0.184 0.268
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Inositol polyphosphates: a new frontier for regulating gene expression.
Alcázar-Román et al. (2008)
7 more

5 mentions without citation

Comparative assessment of strategies to identify similar ligand-binding pockets in proteins.
Govindaraj et al. (2018)
4 more