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Cooperative water filling of a nonpolar protein cavity observed by high-pressure crystallography and simulation.

Collins MD, Hummer G, Quillin ML, Matthews BW, Gruner SM
Proc Natl Acad Sci U S A 102 16668-71 (2005)
Related entries: 2b6w, 2b6x, 2b6y, 2b6z, 2b70, 2b72, 2b73, 2b75, 2oe4

Cited: 101 times
EuropePMC logo PMID: 16269539

Abstract

Formation of a water-expelling nonpolar core is the paradigm of protein folding and stability. Although experiment largely confirms this picture, water buried in "hydrophobic" cavities is required for the function of some proteins. Hydration of the protein core has also been suggested as the mechanism of pressure-induced unfolding. We therefore are led to ask whether even the most nonpolar protein core is truly hydrophobic (i.e., water-repelling). To answer this question we probed the hydration of an approximately 160-A(3), highly hydrophobic cavity created by mutation in T4 lysozyme by using high-pressure crystallography and molecular dynamics simulation. We show that application of modest pressure causes approximately four water molecules to enter the cavity while the protein itself remains essentially unchanged. The highly cooperative filling is primarily due to a small change in bulk water activity, which implies that changing solvent conditions or, equivalently, cavity polarity can dramatically affect interior hydration of proteins and thereby influence both protein activity and folding.

Articles - 2b74 mentioned but not cited (2)

  1. Cooperative water filling of a nonpolar protein cavity observed by high-pressure crystallography and simulation. Collins MD, Hummer G, Quillin ML, Matthews BW, Gruner SM. Proc Natl Acad Sci U S A 102 16668-16671 (2005)
  2. Structural and thermodynamic characterization of T4 lysozyme mutants and the contribution of internal cavities to pressure denaturation. Ando N, Barstow B, Baase WA, Fields A, Matthews BW, Gruner SM. Biochemistry 47 11097-11109 (2008)


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