› 2b83

X-ray diffraction
2.25Å resolution

A single amino acid substitution in the Clostridium beijerinckii alcohol dehydrogenase is critical for thermostabilization

Released:
DOI: 10.2210/pdb2b83/pdb
PDB entry 2b83 coloured by chain, front view.
PDB entry 2b83 coloured by chain, side view.
PDB entry 2b83 coloured by chain, top view.
Source organism: Clostridium beijerinckii
Primary publication:
A single proline substitution is critical for the thermostabilization of Clostridium beijerinckii alcohol dehydrogenase.
Goihberg E, Dym O, Tel-Or S, Levin I, Peretz M, Burstein Y
Proteins 66 196-204 (2007)
PMID: 17063493

Function and Biology Details

Reaction catalysed: 
Propan-2-ol + NADP(+) = acetone + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150631 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP-dependent isopropanol dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 351 amino acids
Theoretical weight: 37.73 KDa
Source organism: Clostridium beijerinckii
Expression system: Escherichia coli
UniProt:
  • Canonical: P25984 (Residues: 1-351; Coverage: 100%)
Gene name: adh
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 79.47Å b: 103.34Å c: 193.307Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.193 0.23
Expression system: Escherichia coli

Quick links

Citations

27 citation in other articles

Improved thermostability of Clostridium thermocellum endoglucanase Cel8A by using consensus-guided mutagenesis.
Anbar et al. (2012)
26 more

1 mention without citation

Function prediction from networks of local evolutionary similarity in protein structure.
Erdin et al. (2013)