› 2b89

Solution NMR

Structural basis for molecular recognition in an affibody:affibody complex

Released:
DOI: 10.2210/pdb2b89/pdb
PDB entry 2b89 coloured by chain, ensemble of 40 models, front view.
PDB entry 2b89 coloured by chain, ensemble of 40 models, side view.
PDB entry 2b89 coloured by chain, ensemble of 40 models, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structural basis for molecular recognition in an affibody:affibody complex.
Lendel C, Dogan J, Härd T
J Mol Biol 359 1293-304 (2006)
PMID: 16750222

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180654 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein A Ig-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 58 amino acids
Theoretical weight: 6.43 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q70AB8 (Residues: 116-173; Coverage: 23%)
Gene name: spa
Sequence domains: B domain
Structure domains: Immunoglobulin FC, subunit C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 93%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Engineered protein scaffolds as next-generation antibody therapeutics.
Gebauer et al. (2009)
7 more

8 mentions without citation

Targeting HER3 using mono- and bispecific antibodies or alternative scaffolds.
Malm et al. (2016)
7 more