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Atomic resolution (0.97 A) structure of the triple mutant (K53,56,121M) of bovine pancreatic phospholipase A2.

Sekar K, Rajakannan V, Gayathri D, Velmurugan D, Poi MJ, Dauter M, Dauter Z, Tsai MD
Acta Crystallogr Sect F Struct Biol Cryst Commun 61 3-7 (2005)
Related entries: 1gh4, 1mks, 1mku, 1o2e, 1o3w, 1une, 1vl9

Cited: 2 times
EuropePMC logo PMID: 16508077

Abstract

The enzyme phospholipase A2 catalyzes the hydrolysis of the sn-2 acyl chain of phospholipids, forming fatty acids and lysophospholipids. The crystal structure of a triple mutant (K53,56,121M) of bovine pancreatic phospholipase A2 in which the lysine residues at positions 53, 56 and 121 are replaced recombinantly by methionines has been determined at atomic resolution (0.97 A). The crystal is monoclinic (space group P2), with unit-cell parameters a = 36.934, b = 23.863, c = 65.931 A, beta = 101.47 degrees. The structure was solved by molecular replacement and has been refined to a final R factor of 10.6% (Rfree = 13.4%) using 63,926 unique reflections. The final protein model consists of 123 amino-acid residues, two calcium ions, one chloride ion, 243 water molecules and six 2-methyl-2,4-pentanediol molecules. The surface-loop residues 60-70 are ordered and have clear electron density.

Articles - 2bax mentioned but not cited (2)

  1. Expression, functional, and structural analysis of proteins critical for otoconia development. Xu Y, Zhang H, Yang H, Zhao X, Lovas S, Lundberg YW. Dev Dyn 239 2659-2673 (2010)
  2. Atomic resolution structure of the double mutant (K53,56M) of bovine pancreatic phospholipase A2. Sekar K, Yogavel M, Gayathri D, Velmurugan D, Krishna R, Poi MJ, Dauter Z, Dauter M, Tsai MD. Acta Crystallogr Sect F Struct Biol Cryst Commun 62 1-5 (2006)




Related citations provided by authors (5)

  1. Atomic resolution (0.97 A) structure of the triple mutant (K53,56,121M) of bovine pancreatic phospholipase A2. Sekar K, Rajakannan V, Gayathri D, Velmurugan D, Poi MJ, Dauter M, Dauter Z, Tsai MD Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 61 3-7 (2005)
  2. Crystal structures of the free and anisic acid bound triple mutant of phospholipase A2.. Sekar K, Vaijayanthi Mala S, Yogavel M, Velmurugan D, Poi MJ, Vishwanath BS, Gowda TV, Jeyaprakash AA, Tsai MD J Mol Biol 333 367-76 (2003)
  3. Observation of additional calcium ion in the crystal structure of the triple mutant K56,120,121M of bovine pancreatic phospholipase A2.. Rajakannan V, Yogavel M, Poi MJ, Jeyaprakash AA, Jeyakanthan J, Velmurugan D, Tsai MD, Sekar K J Mol Biol 324 755-62 (2002)
  4. High-resolution refinement of orthorhombic bovine pancreatic phospholipase A2.. Sekar K, Sundaralingam M Acta Crystallogr D Biol Crystallogr 55 46-50 (1999)
  5. Phospholipase A2 engineering. Structural and functional roles of the highly conserved active site residue aspartate-99.. Sekar K, Yu BZ, Rogers J, Lutton J, Liu X, Chen X, Tsai MD, Jain MK, Sundaralingam M Biochemistry 36 3104-14 (1997)

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