› 2bb6

X-ray diffraction
2Å resolution

Structure of Cobalamin-complexed Bovine Transcobalamin in Monoclinic Crystal Form

Released:
DOI: 10.2210/pdb2bb6/pdb
PDB entry 2bb6 coloured by chain, front view.
PDB entry 2bb6 coloured by chain, side view.
PDB entry 2bb6 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structural basis for mammalian vitamin B12 transport by transcobalamin.
Wuerges J, Garau G, Geremia S, Fedosov SN, Petersen TE, Randaccio L
Proc Natl Acad Sci U S A 103 4386-91 (2006)
PMID: 16537422

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195034 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcobalamin-2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 414 amino acids
Theoretical weight: 46.32 KDa
Source organism: Bos taurus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9XSC9 (Residues: 19-432; Coverage: 100%)
Gene name: TCN2
Sequence domains: Eukaryotic cobalamin-binding protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 4 x CL
Ligand B12 4 x B12
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21
Unit cell:
a: 95.826Å b: 100.664Å c: 99.143Å
α: 90° β: 97.16° γ: 90°
R-values:
R R work R free
0.243 0.241 0.28
Expression system: Komagataella pastoris

Quick links

Citations

19 review citations

Vitamin B12 transport from food to the body's cells--a sophisticated, multistep pathway.
Nielsen et al. (2012)
18 more

8 mentions without citation

Structural basis for mammalian vitamin B12 transport by transcobalamin.
Wuerges et al. (2006)
7 more